Composite

Part:BBa_K2209002

Designed by: Qinyu Ge   Group: iGEM17_Nanjing_NFLS   (2017-10-18)


Fused strain

The function of modified gene verification should be performed. In this present study, the combined plasmid was transferred to expression strain P.putida KT2440 through triphilicity. Donor strain was E.coli DH-5α, E.coli HB101（pRK2013）act as auxiliary strains and recipient bacterium was P.putida KT2440. The fuse bacterium was further using to carry out the verification experiment, it showed higher expression level and could degrade aniline analogue high-efficiency.

Characterization of part BBa_K2209002

Higher expression level and extensive degradation spectrum were obtained in this study. Experiment result showed as follows:

Pic 3.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 165
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1134
    Illegal BsaI.rc site found at 1720
    Illegal SapI site found at 4799


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